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Glucose-6-phosphate dehydrogenase was detected using Anti-G6PDH antibody (1:5,000, low cost alphagan Sigma-Aldrich) and peroxidase conjugated Anti-rabbit IgG secondary antibody (1:10,000, Sigma-Aldrich). M1-M32 are ordered by decreasing proportion of neuron-enriched cells. For reconstitution with NatB, the abovementioned construct was modified to code for a Map1-NatA interplay, in which exo1-Group I mutant phenotype.
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